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KMID : 1123920020160030602
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Korean Journal of Oriental Physiology and Pathology
2002 Volume.16 No. 3 p.602 ~ p.608
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Protective Effect of Puerariae radix Against Ethanol-induced Apoptosis on Human Neuroblastoma Cell Line SK-N-MC
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Koo Gyo-Sung
Cho Son-Hae
Jang Mi-Hyean
Kim Chang-Ju
Kim E-Hwa
Lee Choong-Yeol
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Abstract
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To investigate whether Puerariae radix (PR) possesses protective effect against ethanol (EtOH)-inducecl apoptosis in the central nervous system, MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay, flow cytometric analysis, DNA fragmentation assay. and reverse transcription-polymerase chain reaction (RT-PCR) were performed on human neuroblastoma cell line SK-N-MC. Morphological and biochemical analyses demonstrated that SK-N-MC cells treated with EtOH exhibit classical apoptotic features. On the other hand, cells pre-treated with PR prior to EtOH exposure showed decreased occurrence of classical apoptotic features. In addition, it was shown that PR pre-treatment inhibits EtOH-induced increases in the levels of mRNA expression of bax and caspase-3, while it further enhances the level of bcl-2 expression. These results suggest that PR may exert protective effects against EtOH-induced apoptosis in human neuroblastoma cells.
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KEYWORD
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Puerariae radix, ethanol, apoptosis, neuroblastoma
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